Mouse SPARC Protein 3994

Additional Information:
inaccessionP07214|express systemHEK293|product tagC-His|purity> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC|backgroundSecreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.|molecular weightThe protein has a predicted MW of 33.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.|available size100 g, 500 g|endotoxinLess than 1EU per g by the LAL method.|Mouse SPARC Protein 3994proteinSize and concentration100, 500g and lyophilizedFormLyophilizedStorage InstructionsValid for 12 months from date of receipt when stored at -80C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.Storage bufferShipped at ambient temperature.Purity> 95% as determined by Tris-Bis PAGEtarget relevanceSecreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.Protein namesSPARC (Basement-membrane protein 40) (BM-40) (Osteonectin) (ON) (Secreted protein acidic and rich in cysteine)Gene namesSparc,SparcProtein familySPARC familyMass10090DaFunctionAppears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.Catalytic activityBINDING 273; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000255|PROSITE-ProRule:PRU10142″; BINDING 275; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000255|PROSITE-ProRule:PRU10142″; BINDING 277; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000255|PROSITE-ProRule:PRU10142″; BINDING 279; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000255|PROSITE-ProRule:PRU10142″; BINDING 284; /ligand=”Ca(2+)”; /ligand_id=”ChEBI:CHEBI:29108″; /evidence=”ECO:0000255|PROSITE-ProRule:PRU10142″Subellular locationSecreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane.Post-translational modificationN-glycosylated.Target Relevance information above includes information from UniProt accession: P07214The UniProt Consortium|