Rporation of highly fluorescent DNA base analogue tC: NMR structure and UV-Vis spectroscopy characterization.
Biotin-based assays have been widely applied i n nu clei c aci d s research si nce t he st rong affinity between biotin and streptavidin was discovered in the 1970s.1 The protein-ligand contact is one of the strongest noncovalent interactions, with a Kd of 10- 1 5 M. A biotin label is versatile and is often used to enrich proteins that bind to a specific DNA. Alternatively, biotin offers precise detection, amplification, and/or immobilization of DNA substrates. In addition, the orthogonality of the biotin-streptavidin interaction allows researchers t o u se ot her lab els i n t hei r experiments, without having to worry about cross-reactions. Glen Research’s first biotin product was released in 1991, and our collection has grown substantially since then. Our newest product, 5′-Biotin II phosphoramidite (Figure 1), complements our traditional version I. These two 5′-biotin phosphoramidites differ slightly in terms of the linker, an all-carbon versus an ethylene glycol linker (Figure 1A-B). For those familiar with our amino-modifier offerings, the linkers in versions I and II are derived from amino-modifier C6 and amino-modifier 5, respectively (Figure 1C-D). The addition of 5′-Biotin II phosphoramidite allow s researchers accu st omed t o u si ng the amino-modifier 5 linker to continue to do so in their experiments. Moreover, this new version matches the standard 5′-biotin structure used by major oligonucleotide synthesis providers. The 5′-biotin phosphoramidites offer several advantages to customers:
CAUTION: Even though this structure contains a DMT group, the 5′-Biotin II can only be added once to the 5′-terminus of an oligonucleotide. The DMT group in this structure protects one of the urea nitrogens in the biotin structure and does not enable oligonucleotide elongation, like a normal DMT-O would.
Recent Applications
With the surge of public awareness in disease detection, point-of-care (POC) diagnostic testing is becoming essential to the rapid analysis of patient analytes as it facilitates better diagnosis, monitoring,
and management.69-53-4 custom synthesis POC diagnosis based on nucleic acid testing typically relies on nucleic acid amplification and detection in a single device.112809-51-5 web While PCR is a powerful tool and meets requirements of diagnostic tools, such as specificity, sensitivity, and rapidity, i t req u i res nu merou s ex p eri ment al st ep s, skilled technicians, and costly materials.PMID:30285361 Low-cost tools are of tremendous interest and paper microfluidic devices offer a promising platform to translate isothermal amplification tests to POC diagnostics.2 , 3 The 5′-Biotin II structure was incorporated into oligonucleotides for paper-based devices to detect disease-associated DNA.
A 5′-biotinylated reverse primer and a FAMlabeled forward primer were used in a paperand-plastic device coupled to an isothermal recombinase polymerase amplification (RPA) reaction to detect malarial DNA.3 In this system, the RPA reaction produced a primary product labeled with a biotin tag from the reverse primer. The 5′-FAM forward probe recognized the primary product, resulting in a new secondary probe labeled with both biotin and FAM. The secondary product was detected using lateral flow strips with streptavidin to capture the product on the test line and gold nanoparticles functionalized with anti-FAM to yield a color change in the presence of the target nucleic acid se.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com