Library CCM-2 was plated at a density of about five hundred CFU/cm2 (non-selective circumstances) on LB-agar plates supplemented with 50 mg/ml Spec, 300 mM IPTG, one hundred mg/ml ampicillin and 4 or six mg/ml tazobactam
This phagemid was utilised to transform CJ236 cells which had been then plated on...