390/molecules26195757 Academic Editor: Raffaele Pezzani Received: 21 August 2021 Accepted: 16 September 2021 Published: 23

390/molecules26195757 Academic Editor: Raffaele Pezzani Received: 21 August 2021 Accepted: 16 September 2021 Published: 23 September10 9Department of Medicinal Chemist and Pharmacognosy, College of Pharmacy, Qassim University, Qassim 51452, Saudi Topoisomerase Synonyms Arabia; [email protected] Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11371, Egypt Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia; [email protected] (S.A.A.M.); [email protected] (M.S.M.S.) Division of Biochemistry, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, JUST, Irbid 22110, Jordan Hospital of Police Academy, Nasr city, Cairo 11765, Egypt; mohamedshawky1974@yahoo Division of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, South Valley University, Qena 83523, Egypt; [email protected] Division of Pharmaceutics, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia; [email protected] Department of Clinical Biochemistry, Faculty of Medicine, Umm Al-Qura University, Abdia, Makkah 21955, Saudi Arabia; [email protected] (S.Y.E.); [email protected] (M.Z.E.-R.) Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt; [email protected] Correspondence: [email protected] (H.A.M.); [email protected] (H.M.E.); [email protected] (R.A.K.); Tel.: +00966566176074 (H.A.M.)Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short article is an open access write-up distributed under the terms and circumstances of your Creative Commons Attribution (CC BY) license ( creativecommons.org/licenses/by/ four.0/).Abstract: Sage, Salvia officinalis L., is utilized worldwide as an aromatic herb for culinary purposes as well as a standard medicinal agent for various ailments. Present investigations exhibited the effects of extended dryings from the herb on the yields, composition, oil good quality, and hepatoprotective also as anti-cancer biological activities with the hydrodistillation-obtained essential oils from the aerial parts in the plant. The necessary oils’ yields, compositions, and biological activities levels of the fresh and differently timed and room-temperature dried herbs differed significantly. The lowest yields on the vital oil were obtained from the fresh herbs (FH, 631 mg, 0.16 ), when the highest yield was obtained in the two-week dried herbs (2WDH, 1102 mg, 0.28 ). A notable lower in monoterpenes, with increment in the sesquiterpene constituents, was observed for the FH-based important oil as compared to all the other batches from the essential oils obtained from the differenttimed dried herbs. In addition, characteristic chemotypic constituents of sage, i.e., -pinene, camphene, -pinene, myrcene, 1, 8-cineole, -thujone, and camphor, had been present in significantly higher proportions in all of the dried herbs’ important oils as in comparison to the FH-based essential oil. The in vivo hepatoprotective activity demonstrated considerable reductions in the levels of AST, ALT, and ALP, too as a considerable improve in the total protein (p 0.05) ROCK2 Species contents level, as in comparison with the acetaminophen (AAP) administered experimental group of rats. A substantial reduction (p 0.05) in the ALT level was demonstrated by the 4W