Art in the UMSA-IBMB Diarrheal Disease Project supported by the Swedish Agency for Analysis Economic

Art in the UMSA-IBMB Diarrheal Disease Project supported by the Swedish Agency for Analysis Economic Cooperation (SIDA) (to A.-M.S. and ?S.). E.J. acknowledges monetary help from the Swedish Institute along with the International Science Programme (ISP). We also acknowledge RO1 NIAID AI0094001 funding to T.S. We acknowledge the Texas Advanced Computing Center (TACC) at the University of Texas at Austin for giving high-performance computing sources which have contributed towards the investigation benefits reported in this paper (tacc.utexas.edu).
Phang et al. BMC Complementary and Option Medicine 2013, 13:243 biomedcentral/1472-6882/13/RESEARCH ARTICLEOpen AccessAntioxidant potential, cytotoxic activity and total phenolic content of NPY Y5 receptor Antagonist Synonyms Alpinia pahangensis rhizomesChung-Weng Phang, Sri Nurestri Abd Malek and Halijah IbrahimAbstractBackground: Alpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is employed by the locals to treat flatulence. Within this study, the antioxidant and cytotoxic activities with the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five various cancer and 1 typical cell lines had been investigated. The total phenolic content of every extract and its fractions have been also quantified. This is the very first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract. Techniques: Inside the existing study, the crude methanol and fractionated extract of your rhizomes of Alpinia pahangensis have been investigated for their antioxidant activity using 4 STAT5 Activator Synonyms diverse assays namely, the DPPH scavenging activity, superoxide anion scavenging, -carotene bleaching and minimizing power assays while their phenolic contents had been measured by the Folin-Ciocalteu’s process. In vitro neutral red cytotoxicity assay was employed to evaluate the cytotoxic activity against 5 different cancer cell lines, colon cancer (HCT 116 and HT-29), cervical cancer (Ca Ski), breast cancer (MCF7) and lung cancer (A549) cell lines, and one particular regular cell line (MRC-5). The extract that showed higher cytotoxic activity was further investigated for its chemical constituents by GC-MS (gas chromatography ass spectrometry) analysis. Benefits: The ethyl acetate fraction showed the strongest DPPH radical scavenging (0.35 ?0.094 mg/ml) and SOD activities (51.77 ?four.9 ) whilst the methanol extract showed the highest minimizing energy and also the strongest antioxidant activity within the -carotene bleaching assays in comparison to other fractions. The highest phenolic content material was found inside the ethyl acetate fraction, followed by the crude methanol extract, hexane and water fractions. The outcomes showed a positive correlation among total phenolic content with DPPH radical scavenging capacities and SOD activities. The hexane fraction showed potent cytotoxic effect against KB, Ca Ski and HCT 116 cell lines with IC50 of five.eight ?0.1 and 9.1 ?two.0 ug/ml, respectively. The big components of hexane fraction analysed by GC-MS evaluation were largely methyl esters. Conclusions: The current study suggests that the methanol extract and ethyl acetate fraction of A. pahangensis is really a possible source of all-natural antioxidant for protective also as prevention of life-threatening diseases. The hexane fraction of A. pahangensis might have the prospective to be created into therapeutic choice for treating cancer. Keywords and phrases: Alpinia pahangensis, Antioxidant, Phenolic content material, Cytotoxic activity Correspondence: [email protected] Institute of Biological Scienc.