Y expressed FLAG-tagged human RCAN1.1S protein ( 30 kDa; for extra facts, see Oh et

Y expressed FLAG-tagged human RCAN1.1S protein ( 30 kDa; for extra facts, see Oh et al., 2005; Hoeffer et al., 2007). Reduce blot is stained using a FLAG antibody, confirming expression of transgene. Nse-Cre driver line, CamkII -Cre (T-29) driver line. GAPDH, loading control. Image is representative of three independent blots. WT represents the WT manage for every breeding cross. F, Mice overexpressing human RCAN1 early in improvement (Nse-RCAN1Tg1a) display decreased EPM open-arm time compared with WT littermates. No distinction in open-arm time was observed driving an option RCAN1 construct (RCAN1Tg1) using the very same Nse-Cre driver. No difference in open-arm time was also observed with postdevelopmental RCAN1 overexpression (CamkII -Cre) of either RCAN1 construct. WT-Tg1a (Nse) denotes control RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a Nse-Cre lines. MCP-1/CCL2 Protein Gene ID WT-Tg1 (Nse) denotes handle RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 Nse-Cre lines. WT-Tg1 (CamkII ) denotes manage RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a CamkII -Cre lines. WT-Tg1 (CamkII ) denotes manage RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 CamkII -Cre lines. N 36 Nse-RCAN1Tg1a, 40 WT-Tg1a (Nse), 7 Nse-RCAN1Tg1, 11 WT-Tg1 (Nse), 32 CamkII -RCAN1Tg1a, 38 WT-Tg1a (CamkII ), 11 CamkII -RCAN1Tg1, 17 WT-Tg1 (CamkII ). For some datasets not normally distributed, Mann hitney analysis was utilised. p 0.05, or p 0.01, p 0.001.Hoeffer, Wong et al. ?RCAN1 Modulates Anxiousness and Responses to SSRIsJ. Neurosci., October 23, 2013 ?33(43):16930 ?6944 ?Table 1. OFA activity of mice that overexpress human RCAN1a Time/zone (s) Distance/zone (cm) Genotype Nse-RCAN1 Mean SEM WT-Tg1a (Nse) Imply SEM p worth Nse-RCAN1Tg1 Imply SEM WT-Tg1 (Nse) Imply SEM p value CamkII -RCAN1Tg1a Mean SEM WT-Tg1a (CamkII ) Mean SEM p value CamkII -RCAN1Tg1 Imply SEM WT-Tg1 (CamkII ) Imply SEM p valueTg1aAmbulatory (s) TMPRSS2, Human (P.pastoris, His) Periphery Center Total 62.1 4.3 20.eight 2.4 82.9 4.Resting (s) Periphery Center Total 580.1 13.1 69.9 10.3 650 6.Vertical (s) Periphery Center Total 92 six.7 7.6 1.7 99.six 7.Stereotypic (s) Periphery Center Total 125.three 2.6 25.three 3.3 150.six three.Periphery Center Periphery Center Total 767.6 15.five 132.4 15.5 1576.two 73.four 513.9 55.six 2090.1 81.779.5 120.five 1596.3 510.six 2106.9 61.7 10.eight 10.8 99.7 60.6 128.four 4.9 0.542 0.542 0.87 0.968 0.911 0.952 718.five 35.eight 181.five 35.eight 1633.three 92.1 572.three 78.2 2205.six 140.9 69.eight 9.20.eight 82.five 594.9 2.8 six.two 12.three 0.995 0.956 0.417 19.four 3.1 89.2 11.1 549.five 29.63 658 98.four six.eight 9.5 eight.3 0.591 0.475 0.545 101.7 27.2 651.two 16.1 63.7 9.six.3 104.7 122.8 22.four 145.2 1.two 9.1 3.two 2.1 3.9 0.533 0.669 0.538 0.476 0.284 five.five 2.five three.72 0.7 0.495 8.9 two.four 7 1.four 0.496 1.three 0.6 1.1 0.7 0.835 69.2 ten.3 114.4 four.8 29.eight 5.three 144.two six.732.1 167.9 1551.9 623.0 2174.9 63.0 37.two 37.2 74.7 88.six 105.three 9.2 0.804 0.804 0.502 0.679 0.864 0.622 717.eight 32 746.2 17.9 0.74 799.5 13.2 822.1 10.five 0.191 182.two 32 1802.1 97.1 677.8 75.2 2479.9 128.6 73.128.two 91.3 536.0 106.five 642.5 44.eight five.4 ten.7 27.1 29.5 14.0 eight.1 0.184 0.894 0.737 0.905 0.686 0.139 27.four three.1 one hundred.four six.3 536.9 18.four 85 17.2 621.9 9 77.four 7.48.5 115.four 35.eight 151.two eight.6 7.0 7.1 three.0 0.131 0.904 0.517 0.333 86.3 8.7 131.9 four.1 31.six 3.9 163.five three.153.8 1873.5 706.eight 2580.three 75.1 17.9 89.five 61.7 120.3 four.7 0.74 0.59 0.765 0.57 0.77 100.five 13.two 1736.5 123.six 357.four 43.1 2093.9 137.six 70 6.30 105 546.7 two.eight six.2 12.6 0.535 0.604 0.659 13.3 1.eight 83.3 7 595 14.72.7 619.4 76.4 eight.two 9 7.two 0.512 0.844 0.92 50.7 7.7 645.7 11 50.7 7.83.4 128.7 29.7 158.3 eight.1 two.six 2.7 three.three 0.8.